Published Methodologic Article

A method to quantify infection and colonization of holm oak (Quercus ilex) roots by Phytophthora cinnamomi

Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the “dehesa” ecosystem of south-western Spain. Previously, the focus of studies on P. cinnamomi and holm oak have been on molecular tools for identification, functional responses of the host, together with other physiological and morphological host variables. However, a microscopic index to describe the degree of infection and colonization in the plant tissues has not yet been developed. A colonization or infection index would be a useful tool for studies that examine differences between individuals subjected to different treatments or to individuals belonging to different breeding accessions, together with their specific responses to the pathogen. This work presents a methodology based on the capture and digital treatment of microscopic images, using simple and accessible software, together with a range of variables that quantify the infection and colonization process.


Histopathology of infection and colonization of Quercus ilex fine roots


New article published in the Plant Pathology journal.

We have developed an experience with Q. ilex seedlings, inoculated with Phytophthora cinnamomi (an oomycete responsible of Holm oak disease in south western Iberian Peninsula), in which we have evaluated the development of the pathogen inside the root with histology methodologies, calculating development index with image treatment.

Thanks for all to my thesis directors, and my partner of lab, co-authors of this paper.



Nuevo artículo publicado en Plant Pathology.

Se desarrolló un ensayo sobre plantones de Q. ilex, inoculados con Phytophthora cinnamomi (oomiceto responsable del decaimiento de la encina en el suroeste de la Península Ibérica), en el cual se evaluó el desarrollo del patógeno dentro de la raíz a través del uso de técnicas histológicas, calculando índices de desarrollo con tratamiento de imágenes.

Muchas gracias a mis directores y compañeros de laboratorio, co-autores de éste artículo!